irfp670  (Addgene inc)

Journal: bioRxiv

Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping

doi: 10.1101/2025.03.29.645830

Figure Lengend Snippet: Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear iRFP670 (magenta).

Article Snippet: Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466).

Techniques: Control, Cell Culture, Standard Deviation, Expressing

Journal: bioRxiv

Article Title: 3D Printed Molds for Organ-on-a-Chip and Fluidics: PDMS-Based Rapid and Accessible Prototyping

doi: 10.1101/2025.03.29.645830

Figure Lengend Snippet: Evaluation of MCF10A and 4T1 cell morphology and viability after 72 hours of culture on PDMS slabs cast from 3D printed molds coated with epoxy-acetone 0.5 mixtures, for XTC-3D epoxy (A–C) or Janchun epoxy (D–F) , compared to control PDMS cast from standard 10 cm polystyrene dishes. (A, D) Brightfield and epifluorescence images of MCF10A and 4T1 cells show comparable morphology and confluency between control and 3D printed mold conditions, supporting the biocompatibility of PDMS replicas fabricated from epoxy-coated molds. (B–C, E–F) Bar graphs quantifying viability of MCF10A cells (B, E) and 4T1 (C, F) cells cultured on control vs. 3D printed mold conditions. No significant differences (n.s.) were observed. Data represent mean ± standard deviation. Epifluorescence images in panels A and D show live MCF10A cells expressing cytoplasmic GFP (green) and nuclear RFP (magenta), and 4T1 cells expressing nuclear iRFP670 (magenta).

Article Snippet: Here, we used E2 and M 4T1 clonal cell lines stably expressing near-infrared fluorescent protein (pNLS-iRFP670) and yellow fluorescent protein (YFP) in the nucleus, respectively, which we previously generated to enable live cell fluorescence imaging. pNLS-iRFP670 was a gift from Vladislav Verkhusha (Addgene plasmid # 45466; http://n2t.net/addgene:45466 ; RRID:Addgene_45466).

Techniques: Control, Cell Culture, Standard Deviation, Expressing